Introduction: Dermatophytes are among the common fungal agents implicated in superficial skin infections worldwide. The difficulty in the diagnosis of dermatophytosis relies primarily on the absence of standardization of the collection of clinical specimens and of mycological techniques. Direct examination is essential, as it allows the clinician to start treatment, pending culture. The present study was undertaken with comparative evaluation of microscopic examination and culture media for fungal isolation Methods: Seventy five clinically suspected cases of dermatophytosis were subjected to mycological examination with microscopy and culture using 10% KOH and Sabouraud’s Dextrose Agar (SDA) with cycloheximide. Results: Direct microscopy detected fungal elements in 37 samples from 38 showed positive by culture, the 37 sample which showed negative result by culture method, only 11sample showed negative result by direst microscopy. Conclusion: Difficulty in the diagnosis of dermatophytosis is due to absence of mycological techniques and standardization of collection clinical specimens in addition to lack of commercial availability for most of the reagents needed. Our result and other founding else were created the possibility to clinician to rely on direct microscopical result for treatment of dermatophytosis.